Overview
Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.
Editorials
From The Critics
Reviewer: Alvin Telser, PhD(Northwestern University Feinberg School of Medicine)Description: This book is devoted to a detailed description of the newest microscopy techniques in cellular imaging and application of these techniques to the study of a many features of individual cells.
Purpose: The editor states that this book is intended to provide an authoritative and up-to-date resource for researchers who wish to use the latest in light microscopic techniques. This is an important and timely book and is very worthwhile.
Audience: "The book is intended to be used by experienced or novice researchers. The material in the book is written at a high level and should be very useful to anyone who actually wants to use the techniques described in it. This book is intended for the serious microscopist. The book is divided into four parts and has a total of 24 chapters. The authors of each chapter (about 60 total) are experienced and knowledgeable experts in the field. "
Features: The chapters contain authoritative descriptions and discussion of fluorescence microscopy and fluorescence detectors, laser scanning confocal microscopy, multiphoton excitation fluorescence microscopy, fluorescence resonance energy transfer, spectral microscopy and a few other cutting-edge techniques. The book has a thorough index and is printed on high quality paper that is appropriate for the images that appear in each chapter.
Assessment: This book will be very useful for anyone who plans to use the techniques described in it. It is very up-to-date, and therefore would be of interest to scientists presently using these techniques.
4 Stars! from Doody