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Overview
As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research.
Synopsis
As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research.
Doody Review Services
Reviewer:Omer Iqbal, MD, FACC, FESC(Loyola University Medical Center)
Description:Written by expert researchers, this book presents methods using microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the goal of learning about gene expression, signal transduction, and protein function within living cells. There are adequate black and white illustrations and photographs.
Purpose:The editor hopes that this book will be of interest to all biologists, especially clinicians such as fertility specialists who are interested in applying this powerful method for treatment.
Audience:This book will be quite useful for students, biologists, and clinicians such as fertility experts.
Features:The 15 chapters are written by experts in the field. The book provides excellent informative updates on established techniques such as cRNA expression and examines new, cutting-edge technologies including antisense morpholino oligonucleotides, RNAi for knockdown experiments and the use of integrase to produce transgenic animals, all through microinjection techniques. Each chapter includes a brief introduction, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes providing tips on troubleshooting. All the chapters are well written and are quite informative. The book has adequate black-and-white illustrations and photographs; color illustrations may have provided much clarity in understanding the techniques.
Assessment:This is an informative and useful book for all biologists, particularly fertility specialists. There are not too many books available on this important subject. This one would serve as an ideal resource for new ideas and techniques to aid in the advance of biological research.
Editorials
From The Critics
Reviewer: Omer Iqbal, MD, FACC, FESC(Loyola University Medical Center)Description: Written by expert researchers, this book presents methods using microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the goal of learning about gene expression, signal transduction, and protein function within living cells. There are adequate black and white illustrations and photographs.
Purpose: The editor hopes that this book will be of interest to all biologists, especially clinicians such as fertility specialists who are interested in applying this powerful method for treatment.
Audience: This book will be quite useful for students, biologists, and clinicians such as fertility experts.
Features: The 15 chapters are written by experts in the field. The book provides excellent informative updates on established techniques such as cRNA expression and examines new, cutting-edge technologies including antisense morpholino oligonucleotides, RNAi for knockdown experiments and the use of integrase to produce transgenic animals, all through microinjection techniques. Each chapter includes a brief introduction, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes providing tips on troubleshooting. All the chapters are well written and are quite informative. The book has adequate black-and-white illustrations and photographs; color illustrations may have provided much clarity in understanding the techniques.
Assessment: This is an informative and useful book for all biologists, particularly fertility specialists. There are not too many books available on this important subject. This one would serve as an ideal resource for new ideas and techniques to aid in the advance of biological research.