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Synopsis
Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need. Mycobacteria Protocols, Second Edition updates and refines the methods of the well-received first edition while adding newly developed methods culled from the most cutting-edge research in the field. Ranging from the basics of sub-cellular fractionation to advanced methods using specialized growth conditions and whole genome, transcriptome and proteome analysis, the chapters conform to the Methods in Molecular Biology™ series format, providing step-by-step laboratory protocols, lists of necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Mycobacteria Protocols, Second Edition is the perfect resource to promote and stimulate further research into these intriguing, important and most fractious of bacteria.
Charles E. Edmiston
This book was conceived as a laboratory resource describing in concise detail current molecular techniques used in basic or applied studies involving the mycobacteria. The editors have collected in a single volume numerous molecular protocols, which have been contributed by an outstanding group of international investigators. The spectrum of protocols included encompass laboratory techniques for studying the molecular biology of the mycobacteria to procedures appropriate for diagnostic or epidemiologic studies. This text should be viewed as a valuable resource for any mycobacterial investigator engaged in basic science or clinical studies. It would also be extremely helpful to young investigators or students studying the molecular genetics or microbial pathogenesis of the medically important mycobacteria. The text has been organized into 31 separate chapters. The first third of the book presents several chapters on basic techniques for studying the mycobacteria such as cultivation and preparation of nucleic acid, extraction of RNA, preparation of proteins, and cell-wall fractions. There are several chapters on phage application, transposon mutagenesis, gene replacement, and other techniques for studying gene regulation and expression. The last seven chapters address the use of molecular techniques for laboratory diagnosis and detection of drug resistance. The protocols in each chapter are well documented with concise material and methods sections. The editors have included a helpful "notes" section at the end of each chapter written by the contributors describing their own experiences when using these techniques, including valuable suggestions for overcoming common laboratorypitfalls. This is an excellent resource for either the established or novice investigator. The editors have solicited contributions from over 50 experts, many of whom are recognized as international leaders in the molecular biology and pathogenesis of the mycobacteria. The breadth of techniques covered and the thorough documentation included makes this text an ideal acquisition for either a personal or institutional holding.