Books.org participates in affiliate programs including Bookshop.org and the Amazon Services LLC Associates Program. We may earn a commission from qualifying purchases made through links on this page, at no additional cost to you.
Synopsis
With one-third of the world's population infected with Mycobacterium tuberculosis, over two million people a year dying from tuberculosis, and the appearance of multidrug-resistant strains, the need to understand the biology of M. tuberculosis, and so to develop new interventions, has become acute. In Mycobacterium tuberculosis Protocols, leading investigators with extensive practical knowledge and experience describe their best methods for studying this dangerous pathogen. Packed with step-by-step instructions to ensure successful results, these methods range from basic handling techniques to the application of functional genomics. These molecular techniques are suitable for research in genetics, biochemistry, microbiology, cell biology, epidemiology, and diagnostics, and are at the forefront of biological research as a whole, as well as in focused M. tuberculosis research. Highlights include methods for the basic safety and culture of M. tuberculosis, fractionation of the bacterium (nucleic acids, lipids, culture filtrate, and capsule), the analysis of gene expression (start-site mapping, real-time PCR, microarrays, and proteomics), the growth of the bacterium in macrophages and low oxygen, cytological analysis of the bacteria, and diagnostics. Highly practical and accessible, Mycobacterium tuberculosis Protocols utilizes advanced functional genomics and mutagenesis methodologies to provide both experimental and clinical investigators all the powerful techniques needed to illuminate the molecular biology of tuberculosis and its interactions with host cells, and so drive work on the wide variety of emerging therapeutic opportunities.
Booknews
Provides detailed protocols for studying the molecular biology of the pathogen and its interactions with host cells. Both traditional and novel methodologies are reviewed, with chapters covering everything from safety issues and the storage of strains to the application of the latest functional genomics technologies. These molecular techniques are suitable for research in genetics, cell biology, biochemistry, microbiology, epidemiology, and diagnostics. Techniques include methods for the basic safety and culture of , fractionation of the bacterium, analysis of gene expression, and cytological analysis. Parish is affiliated with St. Bartholomew's and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, UK. Stoker is affiliated with the London School of Hygiene and Tropical Medicine, UK. Annotation c. Book News, Inc., Portland, OR (booknews.com)