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Overview
Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria.Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory.
Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.
Editorials
Trends in Genetics
The book's raison d'etre is as a source of detailed protocols. These are clearly written, and include ideas for experimental controls and useful trouble-shooting notes. Jargon is generally avoided, and the protocols are up to date.…[This] is a much-needed and well-executed attempt at theoretical and practical support to any researcher, established or novice, using phage display.Booknews
Phage display is a rapidly growing field, but the technology has a reputation for requiring considerable technical expertise. This manual aims to provide comprehensive instruction in the theory and applications of phage-display technologies so that any scientist with even modest experience in molecular biology can employ them. In addition to an overview of key aspects of phage display, sections cover the construction, screening, and analysis of antibody libraries on the surface of phage; phage-displayed peptide libraries; and gene- fragment and cDNA-expression libraries. Appendices summarize commonly used experimental procedures, data, recipes, suppliers, and important precautions. Annotation c. Book News, Inc., Portland, OR (booknews.com)Book Details
Published
January 28, 2001
Publisher
Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press, c2001.
Pages
736
Format
Hardcover
ISBN
9780879695460