Genetics - Mapping & Engineering, Genetics - DNA & RNA, Genetics, Biology - Molecular Biology, Biology - Biotechnology, Biotechnology & Bioengineering, Biomedicine, Biochemistry - DNA & RNA

Prins and in Situ Pcr Protocols

Name: Prins and in Situ Pcr Protocols
Author: John R. Gosden
ISBN: 9780896033955

by John R. Gosden

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Overview

Cutting edge researchers demonstrate step-by-step how oligonucleotide primers may be successfully used to detect and amplify or extend complimentary sequences in situ. Through these procedures-often invented by the authors-the door is opened to rapid identification and characterization of chromosomal DNA sequences, viral genomes, and rare messenger RNAs in cells-at hitherto unmatched degrees of sensitivity and specificity. Their innovative techniques-suitable for both novice and experienced researchers-have rapidly become indispensable for many clinical diagnostic procedures, whether in quantification of chromosomes in the identification of aneuploidy for prenatal diagnosis, or in the identification of viral infection in the early stages, or of rare messenger RNAs present in cells.

The book contains predominantly black-and-white illustrations, with some color illustrations.

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Editorials

From The Critics

Reviewer: Philip M. Sass, PhD(University of Illinois at Chicago College of Medicine)
Description: This book is a compilation of PRINS (which is an acronym for oligonucleotide primed in situ hybridization) protocols and their application to various research problems.
Purpose: The PRINS technique is a powerful method combining the sensitivity of PCR with the cytological localization of in situhybridization. The book combines a detailed literature survey and a comprehensive laboratory manual that describes the state of the art in PRINS technology.
Audience: This book is written primarily for those researchers interested in applying molecular cytogenetics and molecular cytology to their research problem.
Features: Many useful and easy-to-follow protocols are covered in great depth as well as examples of the application of PRINS technology to problems as disparate as the physical assignment of sequence tagged sites (which at present cannot be accomplished by purely in situ hybridization techniques) to an estimation of aneuplody rates in human gametes. The structure of the book is a bit baffling, with a well-written introduction to PRINS and related technologies at the end of the book instead of the beginning. Further, many of the chapters contain introductions that are unclear, in particular when detailing the specific application of PRINS to a research problem. However, with few exceptions the protocols are well written and contain sufficient detail for a novice to successfully complete the experiment, although the authors assume some familiarity with basic molecular biology techniques. There are a sufficient number of illustrations in each chapter to allow the protocols to be followed with little effort..
Assessment: I would recommend this book for libraries as well as individual researchers interested in applying PRINS technology.

Philip M. Sass

This book is a compilation of PRINS (which is an acronym for oligonucleotide primed in situ hybridization) protocols and their application to various research problems. The PRINS technique is a powerful method combining the sensitivity of PCR with the cytological localization of in situhybridization. The book combines a detailed literature survey and a comprehensive laboratory manual that describes the state of the art in PRINS technology. This book is written primarily for those researchers interested in applying molecular cytogenetics and molecular cytology to their research problem. Many useful and easy-to-follow protocols are covered in great depth as well as examples of the application of PRINS technology to problems as disparate as the physical assignment of sequence tagged sites (which at present cannot be accomplished by purely in situ hybridization techniques) to an estimation of aneuplody rates in human gametes. The structure of the book is a bit baffling, with a well-written introduction to PRINS and related technologies at the end of the book instead of the beginning. Further, many of the chapters contain introductions that are unclear, in particular when detailing the specific application of PRINS to a research problem. However, with few exceptions the protocols are well written and contain sufficient detail for a novice to successfully complete the experiment, although the authors assume some familiarity with basic molecular biology techniques. There are a sufficient number of illustrations in each chapter to allow the protocols to be followed with little effort.. I would recommend this book for libraries as well as individual researchers interested in applying PRINStechnology.

Booknews

Explains to molecular biologists the step-by-step procedures for using oligonucleotide primers to detect and amplify or extend complimentary sequences in situ, thus allowing the rapid identification and characterization of chromosomal DNA sequences, viral genomes, and rare messenger RNA in cells with a sensitivity and specificity hithertofore unmatched. Includes five color plates. The plastic claw binding lies flat for laboratory use. Annotation c. Book News, Inc., Portland, OR (booknews.com)

3 Stars from Doody