Protein blotting techniques have become common laboratory procedures in the past few years. This text is written by scientists with expertise in these techniques. The versatility of the methods utilizing these procedures have brought about the development of different solid support matrices, as well as a wide variety of protein detection methods. While the most commonly used method in protein blotting is the use of antibodies to detect protein antigens, this technology has been expanded to examine a number of different interactions between proteins and other proteins, as well as other molecules such as carbohydrates and DNA. These methods have further been adapted for amino acid sequencing and purification of proteins for use as immunogens. This book outlines, in detail, numerous protocols and procedures which should help investigators design methods which will be optimal for their specific use.
A guide for professionals and students in the life sciences to a laboratory technique most commonly used to detect protein antigens, but recently also being used to characterize a wide range of different interactions between proteins and other proteins, carbohydrates, and DNA. Describes the equipment, principles, sample preparation, detection methods, and several conventional and innovative applications. Annotation c. Book News, Inc., Portland, OR (booknews.com)
