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Overview
An interdisciplinary panel of expert investigators describe proven methods for investigating the often deadly mechanisms of septic shock. Drawing on insights from a wide variety of fields-ranging from molecular and cellular biology to the physiological measurement of entire animals-these experienced research workers detail the most important techniques for studying sepsis involving endotoxins, toxins derived from Gram-positive organisms, cytokines, nitric oxide and other reactive nitrogen intermediates, and primary cell cultures. They also treat a number of significant techniques that are not well described in the literature, including the the Limulus endotoxin assay, the bioassay of TNF, the purification of bacterial endotoxin and superantigens, and the culture of primary human cells. Multidisciplinary and state-of-the-art, Septic Shock Methods and Prools offers both experimental and clinical investigators a gold-standard collection of key methods for illuminating the basic pathophysiological processes that occur following infection.
The book contains black-and-white illustrations.
Editorials
Charles E. Edmiston
This is a collection of laboratory procedures for investigating the mechanisms of septic shock. Over 30 international investigators have made contributions to this volume on topics ranging from purification of endotoxin to the immunochemical detection of nitric oxide synthase. The text is written as a laboratory manual for investigators engaged in septic shock research. The text has a multidisciplinary appeal to a broad range of scientific investigators in medicine and surgery. Any investigator or student engaged in clinical or experimental studies of the pathophysiologic processes associated with the septic process following infection will benefit from this book. The text has been organized around five separate subject areas: analysis and purification of endotoxin; purification and analysis of streptococcal exotoxin and superantigens; analysis of selected cytokines; analysis of nitric oxide and other oxidant/nitrating agents; and selected cell culture techniques. While the myriad in vitro or in situ techniques for investigating septic shock are not chronicled here, the five areas mentioned are adequately covered, so this would be a useful laboratory reference. I believe that this text is a fine laboratory reference. The section on tissue culture is a bit superficial but it would be difficult, given the space allowed, to include all of the various tissue culture methodologies currently available. Overall, the editor has done an excellent job in the organization of the text and has selected well-qualified investigators who have made a commendable contribution to the various sections of the book. This text is appropriate for a broad-range of interdisciplinary investigators andstudents engaged in septic shock/infection studies.Booknews
Specialists in a range of disciplines from molecular and cellular biology to the physiological measurement of entire animals describe proven methods for investigating the often deadly mechanisms of septic shock. They consider sepsis involving endotoxins, toxins derived from Gram-positive organisms, cytokines, nitric oxide and other reactive nitrogen intermediates, and primary cell cultures. They also treat a number of significant techniques that are not widely described in the literature, including the endotoxin assay, the bioassay of TNR, purifying bacterial endotoxin and superantigens, and culturing primary human cells. Annotation c. Book News, Inc., Portland, OR (booknews.com)From The Critics
Reviewer: Charles E. Edmiston, Jr., PhD(Medical College of Wisconsin)Description: This is a collection of laboratory procedures for investigating the mechanisms of septic shock. Over 30 international investigators have made contributions to this volume on topics ranging from purification of endotoxin to the immunochemical detection of nitric oxide synthase.
Purpose: The text is written as a laboratory manual for investigators engaged in septic shock research. The text has a multidisciplinary appeal to a broad range of scientific investigators in medicine and surgery.
Audience: Any investigator or student engaged in clinical or experimental studies of the pathophysiologic processes associated with the septic process following infection will benefit from this book.
Features: The text has been organized around five separate subject areas: analysis and purification of endotoxin; purification and analysis of streptococcal exotoxin and superantigens; analysis of selected cytokines; analysis of nitric oxide and other oxidant/nitrating agents; and selected cell culture techniques. While the myriad in vitro or in situ techniques for investigating septic shock are not chronicled here, the five areas mentioned are adequately covered, so this would be a useful laboratory reference.
Assessment: I believe that this text is a fine laboratory reference. The section on tissue culture is a bit superficial but it would be difficult, given the space allowed, to include all of the various tissue culture methodologies currently available. Overall, the editor has done an excellent job in the organization of the text and has selected well-qualified investigators who have made a commendable contribution to the various sections of the book. This text is appropriate for a broad-range of interdisciplinary investigators and students engaged in septic shock/infection studies.
3 Stars from Doody