Structure-Function Analysis of G Protein-Coupled Receptors
Jurgen Wess (Editor), Jurgen Wess, J rgen WessBooks.org participates in affiliate programs including Bookshop.org and the Amazon Services LLC Associates Program. We may earn a commission from qualifying purchases made through links on this page, at no additional cost to you.
Overview
G protein-coupled receptors (GPCRs) are the largest single class of receptors in biology, often playing key roles in a remarkably large number of physiological and pathophysiological conditions. GPCRs or GPCR-dependent signalling pathways are the targets of a very large number of therapeutically useful drugs. Detailed knowledge about the molecular structure of GPCRs should therefore pave the way for the design of novel drugs with increased efficacy and specificity.
This volume provides a concise, up-to-date presentation of methods (including molecular genetic, biochemical, and biophysical) which have been used successfully in studying the structure and function of GPCRs. With contributions from international leaders in the field, the editor provides overviews of various techniques, followed by in-depth descriptions of basic procedures and discussions of critical experimental parameters.
Divided into specific, accessible sections, Structure-Function Analysis of G Protein-Coupled Receptors includes:
* An overview of mutagenesis techniques
* Examples of molecular modeling techniques
* Using peptides as tools for the study of GPCR interactions
* Site-Directed Spin Labeling (SDSL) studies of the GPCR rhodopsin
* A complete description of the electron-crystalographic analysis of two-dimensional rhodopsin crystals
* The use of nuclear magnetic resonance techniques to study GPCR structure
The book contains predominantly black-and-white illustrations, with some color illustrations.
Synopsis
Structure-Function Analysis of G Protein-Coupled Receptors edited by Jürgen Wess.
G protein-coupled receptors (GPCRs) are the largest single class of receptors in biology, playing key roles in a remarkably large number of physiological and pathophysiological conditions. GPCRs or GPCR-dependent signalling pathways are the targets of a very large number of therapeutically useful drugs. Detailed knowledge about the molecular structure of GPCRs should therefore pave the way for the design of novel drugs with increased efficacy and specificity.
This volume provides a concise, up-to-date presentation of methods (including molecular genetic, biochemical, and biophysical) which have been used successfully in studying the structure and function of GPCRs. With contributions from international leaders in the field, the editor provides overviews of various techniques, followed by in-depth descriptions of basic procedures and discussions of critical experimental parameters.
Divided into specific, accessible sections, Structure-Function Analysis of G Protein-Coupled Receptors includes:
* An overview of mutagenesis techniques.
* Examples of molecular modeling techniques.
* Using peptides as tools for the study of GPCR interactions.
* Site-Directed Spin Labeling (SDSL) studies of the GPCR rhodopsin.
* A complete description of the electron-crystallographic analysis of two-dimensional rhodopsin crystals.
* The use of nuclear magnetic resonance techniques to study GPCR structure.
Structure-Function Analysis of G Protein-Coupled Receptors is an invaluable reference for receptor biochemists and biological chemists, pharmacologists, and neuroscientists as well as molecular biologists, cell biologists, and structural biologists worldwide.
Also in this series: Receptor Localization:
* Laboratory Methods and Procedures edited by Marjorie A. Ariano.
* Identification and Expression of G Protein-Coupled Receptors edited by Kevin R. Lynch.
Myron L. Toews
This volume of the Receptor Biochemistry and Methodology Series is a current and comprehensive overview of the diverse experimental approaches used to investigate the structures of G protein-coupled receptors and the functional correlates of specific structures. There are hundreds of G protein-coupled receptors, dozens of which are already targets of clinically useful drugs, and these receptors remain important targets for new drug development. A detailed understanding of the structures and functions of these receptors is thus an important goal of much current research. This volume is targeted to research scientists, primarily to those already working with a G protein-coupled receptor and interested in identifying specific structural features and related functions for their receptor of interest. In each of the 17 chapters, a different experimental approach is described. These are written by individual authors with extensive experience utilizing that approach. The editor does an excellent job of providing a general introduction to the various strategies and their rationale, more detailed descriptions of specific methodologies and their successful application to one receptor or another, and information on the current status of our knowledge in this area. In about half of the chapters the contributors deal with various applications of specific site-directed mutagenesis and genetic techniques and subsequent functional analyses, and about half deal with instrumental analytic approaches such as NMR, crystallography, and fluorescence spectroscopy. In the final chapter, several successes in new drug development utilizing these approaches are presented. There are the expected variationsin style and depth of presentation between chapters; some contributors present detailed experimental protocols used for one specific receptor, and others focus on general principles and strategies with little experimental detail; however, each of the chapters is easy to read and highly informative. This book is excellent reading for anyone interested in exploring the diverse approaches available for structure-function analysis of G protein-coupled receptors.
Editorials
From The Critics
Reviewer: Myron L. Toews, PhD(University of Nebraska Medical Center)Description: This volume of the Receptor Biochemistry and Methodology Series is a current and comprehensive overview of the diverse experimental approaches used to investigate the structures of G protein-coupled receptors and the functional correlates of specific structures.
Purpose: There are hundreds of G protein-coupled receptors, dozens of which are already targets of clinically useful drugs, and these receptors remain important targets for new drug development. A detailed understanding of the structures and functions of these receptors is thus an important goal of much current research.
Audience: This volume is targeted to research scientists, primarily to those already working with a G protein-coupled receptor and interested in identifying specific structural features and related functions for their receptor of interest.
Features: In each of the 17 chapters, a different experimental approach is described. These are written by individual authors with extensive experience utilizing that approach. The editor does an excellent job of providing a general introduction to the various strategies and their rationale, more detailed descriptions of specific methodologies and their successful application to one receptor or another, and information on the current status of our knowledge in this area. In about half of the chapters the contributors deal with various applications of specific site-directed mutagenesis and genetic techniques and subsequent functional analyses, and about half deal with instrumental analytic approaches such as NMR, crystallography, and fluorescence spectroscopy. In the final chapter, several successes in new drug development utilizing these approaches are presented. There are the expected variations in style and depth of presentation between chapters; some contributors present detailed experimental protocols used for one specific receptor, and others focus on general principles and strategies with little experimental detail; however, each of the chapters is easy to read and highly informative.
Assessment: This book is excellent reading for anyone interested in exploring the diverse approaches available for structure-function analysis of G protein-coupled receptors.
Myron L. Toews
This volume of the Receptor Biochemistry and Methodology Series is a current and comprehensive overview of the diverse experimental approaches used to investigate the structures of G protein-coupled receptors and the functional correlates of specific structures. There are hundreds of G protein-coupled receptors, dozens of which are already targets of clinically useful drugs, and these receptors remain important targets for new drug development. A detailed understanding of the structures and functions of these receptors is thus an important goal of much current research. This volume is targeted to research scientists, primarily to those already working with a G protein-coupled receptor and interested in identifying specific structural features and related functions for their receptor of interest. In each of the 17 chapters, a different experimental approach is described. These are written by individual authors with extensive experience utilizing that approach. The editor does an excellent job of providing a general introduction to the various strategies and their rationale, more detailed descriptions of specific methodologies and their successful application to one receptor or another, and information on the current status of our knowledge in this area. In about half of the chapters the contributors deal with various applications of specific site-directed mutagenesis and genetic techniques and subsequent functional analyses, and about half deal with instrumental analytic approaches such as NMR, crystallography, and fluorescence spectroscopy. In the final chapter, several successes in new drug development utilizing these approaches are presented. There are the expected variationsin style and depth of presentation between chapters; some contributors present detailed experimental protocols used for one specific receptor, and others focus on general principles and strategies with little experimental detail; however, each of the chapters is easy to read and highly informative. This book is excellent reading for anyone interested in exploring the diverse approaches available for structure-function analysis of G protein-coupled receptors.Booknews
Intended for receptor biochemists, biological chemists, pharmacologists, neuroscientists, molecular biologists, cell biologists, and structural biologists. Discusses the structure and function of G protein-coupled receptors (GPCRs) which are the targets of many theraputic drugs. Topics discussed in 17 essays include mutagenesis techniques, molecular modeling techniques, the use of peptides as tools to study GPCR interactions, and the use of nuclear magnetic resonance techniques to study GPCR structure, among others. Annotation c. Book News, Inc., Portland, OR (booknews.com)4 Stars! from Doody