Transgenic Animal Technology: A Laboratory Handbook

Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols New section on analysis

Audience: Students, postdoctoral fellows, and researchers at academic and industrial/agricultural laboratories using non-traditional transgenic models in such areas as molecular and cell biology, developmental biology, neuroscience, pharmacology, biophysiology, cardiology, oncology and genetics.

Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer—from molecular methods to whole animal considerations—for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems.

Key Features
• Contains a comprehensive collection of transgenic animal and gene transfer methods
• Teaches practical step-by-step protocols
• Covers a range of laboratory and domestic animal species
• Describes existing methods of gene transfer
• Discusses molecular analytical techniques and considerations
• Illustrated with useful tables, charts and diagrams

New Contributions to the Second Edition:
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• An entire section devoted to molecular biology, analyses and enabling technologies.
• Nuclear transfer (cloning) in laboratory and domestic animals
• Gene targeting and conditional modeling in embryonic stem cells
• Retrovirus-mediated gene transfer
• State-of-the-art techniques for production of transgenic fish, mice,
nonhuman primates, poultry, rabbits, rats, ruminants, and swine
• Assisted reproductive technologies and bioinformatics based resources

The Second Edition contains 85% new content!

Booknews

A well-illustrated handbook that covers the technical aspects of gene transfer in animals--from molecular methods to whole animal considerations--for the important laboratory and domestic animal species. It describes methodologies employed by leading laboratories, and is suitable as a resource for researchers as well as a tool for training technicians and students. Metal comb binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Dr. Pinkert is an Associate Professor in the Department of Comparative Medicine and Director of the Transgenic Animal/ES Cell Resource at the University of Alabama at Birmingham. He earned his B.S. from Colorado State University, M.S. from Southern Illinois, and Ph.D. in 1983 from the University of Georgia, followed by postdoctoral training at the University of Pennsylvania. He was an Assistant Professor at the University of Missouri and then worked at DNX, Inc. (where his proposal for the National Transgenic Development Facility for the National Institute of Child Health and Human Development was awarded and implemented) before joining the UAB faculty. Dr. Pinkert's primary research efforts involve gene transfer, expression, and regulation using transgenic animal modeling. His work has illustrated the potential of modifying the immune system, enhancing growth performance, and the feasibility of molecular farming. He has published extensively on transgenic modeling, reproductive biology, and embryology of laboratory and domestic animals. He serves as an Editro of Transgenic Research and has served as an ad hoc reviewer or consultant for several journals, government agencies, and companies.