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Overview
Over the past decades, the pathogenesis, diagnosis, treatment and prevention of cardiovascular diseases have been benefited significantly from intensive research activities. In order to provide a comprehensive "manual" in a field that has become as broad and deep as cardiovascular medicine, Vascular Biology Protocols covers a wide spectrum of in vivo and in vitro techniques encompassing biochemical, pharmacological and molecular biology disciplines which are currently used to assess vascular disease progression. Each chapter included in this volume focuses on a specific vascular biology technique and describes various applications as well as caveats of these techniques. The protocols included here are described in detail, allowing beginners with little experience in the field of vascular biology to embark on new research projects.Synopsis
Over the past decades, the pathogenesis, diagnosis, treatment and prevention of cardiovascular diseases have been benefited significantly from intensive research activities. In order to provide a comprehensive "manual" in a field that has become as broad and deep as cardiovascular medicine, Vascular Biology Protocols covers a wide spectrum of in vivo and in vitro techniques encompassing biochemical, pharmacological and molecular biology disciplines which are currently used to assess vascular disease progression. Each chapter included in this volume focuses on a specific vascular biology technique and describes various applications as well as caveats of these techniques. The protocols included here are described in detail, allowing beginners with little experience in the field of vascular biology to embark on new research projects.
Comprehensive and easy-to-use, Vascular Biology Protocols will prove valuable for all those who wish to broaden their knowledge of cardiovascular research tools.
Doody Review Services
Reviewer:Vera P. Shively, BS, MS(Northwestern University Feinberg School of Medicine)
Description:This book of detailed protocols for researchers in the field of vascular biology is particularly relevant for scientists using animal and cell culture models.
Purpose:The purpose is to provide a comprehensive manual for in vivo and in vitro techniques commonly used to study the progression of cardiovascular disease. A book such as this one was needed and it meets the authors' objectives.
Audience:Researchers new to the techniques commonly used to study cardiovascular disease, including clinician scientists, basic research scientists, graduate students, or residents are the intended audience. Although the book is written for beginners, even experienced researchers would find techniques that are new to them.
Features:The book includes protocols for animal models, ex vivo organ systems and relevant functional studies, cell culture isolations, and pertinent experiments. In addition, it presents protocols for basic as well as sophisticated histology, along with instructions for the assessment of results. The final chapter includes methodology for stem cell therapy. For researchers in vascular biology, the strength of this book is the relevance of the protocols and the detail in which they are described. The sections on materials and methods are the most comprehensive I've seen, including separate lists for solutions, supplies, and surgical tools, as well as extensive notes and references on the nuances of each technique. The protocols for animal surgery are particularly thorough. One of the shortcomings of the book is paradoxically one of its assets -- the "see Notes" feature. Basically, it is an example of the limitations, and sometimes awkwardness, of print in the age of electronic text with links. The "see Notes" feature augments each chapter with relevant information, but it is a bit of a nuisance to flip back and forth through the pages.
Assessment:This would be a valuable addition to any laboratory doing research in vascular biology. It is small in size, but substantial in its usefulness to researchers in this area of study. It is not a replacement for the Current Protocols series from John Wiley & Sons, but could be considered a complement to this series as an initial resource for protocols with a narrower focus.
Editorials
From The Critics
Reviewer: Vera P. Shively, BS, MS(Northwestern University Feinberg School of Medicine)Description: This book of detailed protocols for researchers in the field of vascular biology is particularly relevant for scientists using animal and cell culture models.
Purpose: The purpose is to provide a comprehensive manual for in vivo and in vitro techniques commonly used to study the progression of cardiovascular disease. A book such as this one was needed and it meets the authors' objectives.
Audience: Researchers new to the techniques commonly used to study cardiovascular disease, including clinician scientists, basic research scientists, graduate students, or residents are the intended audience. Although the book is written for beginners, even experienced researchers would find techniques that are new to them.
Features: "The book includes protocols for animal models, ex vivo organ systems and relevant functional studies, cell culture isolations, and pertinent experiments. In addition, it presents protocols for basic as well as sophisticated histology, along with instructions for the assessment of results. The final chapter includes methodology for stem cell therapy. For researchers in vascular biology, the strength of this book is the relevance of the protocols and the detail in which they are described. The sections on materials and methods are the most comprehensive I've seen, including separate lists for solutions, supplies, and surgical tools, as well as extensive notes and references on the nuances of each technique. The protocols for animal surgery are particularly thorough. One of the shortcomings of the book is paradoxically one of its assets — the "see Notes" feature. Basically, it is an example of the limitations, and sometimes awkwardness, of print in the age of electronic text with links. The "see Notes" feature augments each chapter with relevant information, but it is a bit of a nuisance to flip back and forth through the pages. "
Assessment: This would be a valuable addition to any laboratory doing research in vascular biology. It is small in size, but substantial in its usefulness to researchers in this area of study. It is not a replacement for the Current Protocols series from John Wiley & Sons, but could be considered a complement to this series as an initial resource for protocols with a narrower focus.