Adipose Tissue Protocols

Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a broad range of techniques, including the choice of adipose tissue depot and of morphological techniques for work on both brown adipose tissue (BAT) and white adipose tissue (WAT). Major treatment is accorded to the isolation, subcellular fractionation, and transfection of low density adipocytes as well as metabolic aspects of nutrient uptake and assays of nutrient and ion fluxes. Innovative and highly practical, Adipose Tissue Protocols offers endocrinologists, physiologists, cell biologists, and pharmacologists a gold-standard collection of proven methods for effective research on adipose tissue from the nutritional to the physiological and the molecular levels.

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Adipose tissue is now recognized as a widely-dispersed secretory organ that plays a significant role in obesity. Ailhaud (U. de Nice-Sophia Antipolis, Nice, France) and a team of European and US laboratory experts and clinicians detail the classification, key techniques employed, and their potential problems, in the in vivo and in vitro study of brown and white fat tissues and cells from the nutritional, physiological, and molecular levels. Major treatment is accorded to the isolation, subcellular fractionation, and gene transfer into mature adipocytes, as well as metabolic aspects. Illustrations include microscopy study results, and a man wearing a portable detector for measuring adipose tissue blood flow. Annotation c. Book News, Inc., Portland, OR (booknews.com)