Gene Cloning and Manipulation

Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.

Incl. genomic libraries/the polymerase chain reaction/use of cloned DNA/Gram-positive & Gram-negative bacteria/fungi/etc.

Fully updated edition of this concise guide to recombinant DNA techniques for advanced undergraduates.

Christopher Howe is Professor of Plant and Microbial Biochemistry at the University of Cambridge. His research interests include biochemistry and molecular biology of photosynthetic organisms, and the evolutionary processes that gave rise to them. He has taught molecular biology for twenty years.