Genetics - Mapping & Engineering, Genetics, Biology - Molecular Biology, Genetics - General and Miscellaneous, Biochemistry - General & Miscellaneous, Biology - Biotechnology
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Overview
Receptor Cloning:S.C. Sealfon, Approaches to Receptor Cloning.
D.I. Schuster and R.B. Murphy, Purification of Receptors.
M.J. Walsh, Protein and Peptide Microsequencing: Applications in Neuroscience and Receptor Research.
R.D. Zuhlke, H.-J. Zhang, and R.H. Joho, Xenopus Oocytes: A System for Expression Cloning and Structure--Function Studies of Ion Channels and Receptors.
D.K. Grandy, Q.-Y. Zhou, C. Bouvier, C. Saez, and J.R. Bunzow, Cloning of G Protein-Coupled Opioid Receptors Using Degenerate PCR and Low Stringency Homology Screening.
M. Tsutsumi and B. Gillo, Hybrid Arrest Screening in Oocytes.
E.F. Kirkness and J.C. Venter, Receptor Cloning: High-Throughput Sequencing of cDNA Tags for Identification of Novel Genes.
Expression and Characterization:
R.P. Millar, J. Davidson, C. Flanagan, and I. Wakefield, Ligand Binding and Second Messenger Assays for Cloned Gq/G11-Coupled Neuropeptide Receptors: The GnRH Receptor.
R.L. Neve and K.A. Neve, Receptor Expression in Mammalian Cells. M. Cascio, Baculovirus Expression of Receptors and Channels.
J.A. Salon and J.C. Owicki, Real-Time Measurements of Receptor Activity: Applications of Microphysiometric Techniques to Receptor Biology.
K.A. Neve, M.P. Rosser, and D.L. Barber, Regulation of Na+-H+ Exchange by G Protein-Coupled Receptors.
P.J.S. Stork, A. Misra-Press, and M.-G. Pan, Receptor-Activated Tyrosine Phosphatases: Activity Assays and Molecular Cloning.
Studies of Functional Domains of Receptor and Channels:
T.M. Fong, M.R. Candelore, and C.D. Strader, Site-DirectedMutagenesis.
S. Suryanarayana and B.K. Kobilka, Receptor Chimers.
D. Palm, G. Munch, and D. Malek, Mapping G Protein Coupling Domains by Site-Specific Peptides.
C.J.L. Carruthers and T.P. Sakmar, Synthesis and Expression of Synthetic Genes: Applications to Structure-Function Studies of Receptors.
J. Hoebeke, J.-G. Guillet, and A.D. Strosberg, Use of Receptors Expressed in Escherichia coli to Study Autoimmunity against G Protein-Coupled Membrane Proteins.
J.A. Ballesteros and H. Weinstein, Integrated Methods for the Construction of Three Dimensional Models and Computational Probing of Structure-Function Relations in G-Protein Coupled Receptors.
Localization and Regulation:
B.J. Ciliax, C. Heilman, S. Edmunds, S.M. Hersch, and A.I. Levey, Anti-fusion Protein Antibodies Specific for Receptor Subtypes.
M.A. Ariano and D.R. Sibley, Development of Antireceptor Antibodies Using Synthetic Peptides.
M. Jakubowski, Receptor mRNA Measurement by Multiplex Nuclease Protection Assay.
M. Shih, C.M. Moxham, and C.C. Malbon, Antisense DNA/RNA-Based Strategies to Analysis of Signal Transduction via G Proteins.
Index.
The book contains black-and-white illustrations.
Editorials
From The Critics
Reviewer: Chad A. Ellis(Rush Medical College of Rush University)Description: This book provides modern biochemical and molecular biology methods to characterize receptors. Using specific examples and detailed instructions, it offers many options by suggesting the most current and common techniques used in laboratories everyday.
Purpose: In this era of rapid cloning of the human genome, the uncovering of receptor DNA sequences allows for transition to the next step in receptor biology. This would include further insights into receptor structure, control, and function. This volume concentrates on the latest methods used following the elucidation of a receptors sequence, namely computational modeling, structure-function studies, localization, and regulation. This is necessary for those laboratories seriously undertaking receptor biology research on any scale.
Audience: This book is suitable for all persons involved in receptor-related research. The author (and contributors) are well represented in receptor-related literature.
Features: The book contains an adequate mix of cartoons depicting structures, flow charts, photographs, and actual data. The references are current and numerous. The overall appearance is much like a lab manual in textbook format. An appreciated feature is the numerous and complete procedures that are accompanied by comments.
Assessment: When combined with a basic molecular biology lab manual, this edition is a necessary addition for use in current strategies in receptor characterization. I highly recommend this volume for purchase by a bookstore, library, or researcher.
Chad A. Ellis
This book provides modern biochemical and molecular biology methods to characterize receptors. Using specific examples and detailed instructions, it offers many options by suggesting the most current and common techniques used in laboratories everyday. In this era of rapid cloning of the human genome, the uncovering of receptor DNA sequences allows for transition to the next step in receptor biology. This would include further insights into receptor structure, control, and function. This volume concentrates on the latest methods used following the elucidation of a receptors sequence, namely computational modeling, structure-function studies, localization, and regulation. This is necessary for those laboratories seriously undertaking receptor biology research on any scale. This book is suitable for all persons involved in receptor-related research. The author (and contributors) are well represented in receptor-related literature. The book contains an adequate mix of cartoons depicting structures, flow charts, photographs, and actual data. The references are current and numerous. The overall appearance is much like a lab manual in textbook format. An appreciated feature is the numerous and complete procedures that are accompanied by comments. When combined with a basic molecular biology lab manual, this edition is a necessary addition for use in current strategies in receptor characterization. I highly recommend this volume for purchase by a bookstore, library, or researcher.5 Stars! from Doody
Book Details
Published
March 22, 1995
Publisher
Academic Press Inc
Pages
519
Format
Textbook Hardcover
ISBN
9780121852955