Overview
Completion of sequencing of the human genome, as well as those of many other organisms, has now opened the door for exploration of the proteome, the many thousands of interacting proteins in a given organism. Critical to tackling the complexity of the proteome is a workable strategy using reliable and tested protocols for identifying, isolating, and quantifying proteins in cells and cell pathways and for performing functional assays. Proteins and Proteomics: A Laboratory Manual, authored by Richard Simpson of the Ludwig Institute for Cancer Research, Melbourne, provides the first authoritative and wide-ranging protocol-based approach to proteomics. Presented as a logical strategy for analyzing proteomes, Proteins and Proteomics provides information about protein structure and numerous methods for the preparation and analysis of protein samples ranging from electrophoresis and mass spectrometry to protein chips and informatics. Extensive background information and references are provided regarding the theoretic aspects of the techniques presented as well as their applications. This manual is an essential laboratory tool for genetics and molecular biology investigators moving from studies of genomics and genotype to those of proteomics and phenotype. It is invaluable for cell biologists, developmental biologists, and neuroscientists exploring the intricacies of the proteome. It will also be an excellent resource for protein chemists and biochemists.Synopsis
Written for both graduate students and experienced investigators, this manual describes the general principles, analytical methods, and protocols involved in the study of proteomics. Simpson (Joint Proteomics Laboratory, Australia) begins with polyacrylamide gel electrophoresis for the separation of proteins in complex mixtures and the preparation of cellular extracts. Further protocols are provided for reversed-phase liquid chromatography, amino- and carboxyl-terminal sequence analysis, and peptide mapping. The largest chapter consists of 13 protocols for using mass spectrometry to identify proteins. Each protocol lists the reagents and equipment required and step-by-step instructions for performing the method. Annotation c. Book News, Inc., Portland, OR
Proteins and Proteomics: A Laboratory Manual is an invaluable information tool both for the experienced protein chemist who bravely ventures into the new world of proteomics and for the novice to proteins and proteomes. By focusing on what is currently considered the bedrock of proteomics technologies, Professor Simpson ensures that-in spite of the rapid advances that characterize contemporary proteomics research-this volume will remain relevant and current for years to come.