Genetics - Mapping & Engineering, Immunology
Antibody Engineering: A Practical Approach (Practical Approach Series)
McCafferty, Hoogenboom, John, Hennie R.
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Overview
Recombinant DNA techniques have revolutionized the isolation and production of antibodies. This volume describes methods and technologies which will allow the researcher to isolate a new antibody, analyse its properties, format the correct antibody or fragment, and produce sufficient quantities for experimental use. Topics in this volume include: generation and analysis of antibodies; antibody repertoires; antibody screening and selection; measuring antibody affinities; sequence analysis; antibody engineering and production; conversion of rodent antibodies to human antibodies by CDR grafting or guided selection; choosing and optimizing effector functions; preparation and use of antibody-based molecules in eukaryotic and prokaryotic systems; scaling up manufacture; and generation of high-affinity high-specificity human antibodies in appropriate formats. Antibody Engineering is a unique manual of recombinant DNA methods for all those working with antibodies in research, diagnostics, and therapeutics. Each chapter is written by a leading researcher in the field and provides essential background information, fully tested protocols, sample data from using these methods, trouble-shooting comments, and key hints and tips for success.The book contains black-and-white illustrations.
Editorials
From The Critics
Reviewer: Katalin Mikecz, MD, PhD(Rush Medical College of Rush University)Description: This volume of The Practical Approach Series provides strategies and up-to-date protocols for the design and production of monoclonal antibodies using recombinant DNA technology.
Purpose: Topics include analysis and construction of antibody gene repertoires; transfer and manipulation of human antibody repertoires in transgenic mice; expression of immunoglobulin genes in mammalian cells; large-scale production of antibody fragments in prokaryotes; conversion of rodent into human antibodies by CDR grafting and guided selection; measurement and manipulation of antibody affinities; and optimization of antibody effector functions.
Audience: The book is an indispensable manual for researchers wishing to improve the qualities of monoclonal antibodies for specific research applications and for those who seek advanced and proven methods to generate high-affinity, high-specificity human antibodies for in vivo therapeutic use.
Features: Written by internationally recognized experts in the field, each chapter contains sufficient background information explaining the principles of pertinent methodological approaches and provides up-to-date, fully tested, step-by-step protocols. These are supplemented with useful germline and primer sequence information and extensive referencing.
Assessment: This resource is highly recommended for libraries and bookstores of academic, medical technology, and pharmaceutical institutions.
Katalin Mikecz
This volume of The Practical Approach Series provides strategies and up-to-date protocols for the design and production of monoclonal antibodies using recombinant DNA technology. Topics include analysis and construction of antibody gene repertoires; transfer and manipulation of human antibody repertoires in transgenic mice; expression of immunoglobulin genes in mammalian cells; large-scale production of antibody fragments in prokaryotes; conversion of rodent into human antibodies by CDR grafting and guided selection; measurement and manipulation of antibody affinities; and optimization of antibody effector functions. The book is an indispensable manual for researchers wishing to improve the qualities of monoclonal antibodies for specific research applications and for those who seek advanced and proven methods to generate high-affinity, high-specificity human antibodies for in vivo therapeutic use. Written by internationally recognized experts in the field, each chapter contains sufficient background information explaining the principles of pertinent methodological approaches and provides up-to-date, fully tested, step-by-step protocols. These are supplemented with useful germline and primer sequence information and extensive referencing. This resource is highly recommended for libraries and bookstores of academic, medical technology, and pharmaceutical institutions.5 Stars from Doody
Book Details
Published
June 30, 1996
Publisher
Oxford University Press
Pages
325
Format
Hardcover, 1996
ISBN
9780199635931