Antibody Engineering

Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years. This has resulted in rapid changes in how to handle antibodies for research. This new Practical Approach volume responds to this change by assembling, in one volume, protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities. The book is divided into two parts: the first describes the generation and analysis of antibodies and the second covers engineering and production. This timely book will be of interest to those involved in both clinical and molecular biological applications of antibodies.

The book contains black-and-white illustrations.

Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years. This has resulted in rapid changes in how to handle antibodies for research. This new Practical Approach volume responds to this change by assembling, in one volume, protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities. The book is divided into two parts: the first describes the generation and analysis of antibodies and the second covers engineering and production. This timely book will be of interest to those involved in both clinical and molecular biological applications of antibodies.

Katalin Mikecz

This volume of The Practical Approach Series provides strategies and up-to-date protocols for the design and production of monoclonal antibodies using recombinant DNA technology. Topics include analysis and construction of antibody gene repertoires; transfer and manipulation of human antibody repertoires in transgenic mice; expression of immunoglobulin genes in mammalian cells; large-scale production of antibody fragments in prokaryotes; conversion of rodent into human antibodies by CDR grafting and guided selection; measurement and manipulation of antibody affinities; and optimization of antibody effector functions. The book is an indispensable manual for researchers wishing to improve the qualities of monoclonal antibodies for specific research applications and for those who seek advanced and proven methods to generate high-affinity, high-specificity human antibodies for in vivo therapeutic use. Written by internationally recognized experts in the field, each chapter contains sufficient background information explaining the principles of pertinent methodological approaches and provides up-to-date, fully tested, step-by-step protocols. These are supplemented with useful germline and primer sequence information and extensive referencing. This resource is highly recommended for libraries and bookstores of academic, medical technology, and pharmaceutical institutions.

McCafferty, John; Hoogenboom, Hennie; Chiswell, Dave

The contributors represent the specialties of biochemistry, molecular genetics, molecular oncology, and biology. Most are from research institutes and universities in the U.S., the U.K., France, Germany, Switzerland, and Norway. Institutions prominently represented include Ludwig Institute for Cancer Research, Institut Pasteur, Cambridge Antibody Technology, Ltd., Universitat Zurich, and Beckman Research Institute of the City of Hope.